2X General Assay Diluent is a mammalian protein-based ELISA additive used to equalize the sample and standard matrices for a more accurate result. Large differences in matrix complexity environment between standard curve and sample wells are the main cause for target analyte under-recovery problems in sandwich ELISA analysis. General Assay Diluent helps equalize matrix complexity disparity issues, reduces non-specific interactions between sample proteins and the plate, and reduces interference from complement and thrombin present in serum and plasma samples. Our 2X concentrated version is helpful for shipping purposes and produces less waste.