CFSE Green Fluorescent Stain - for cell labelling and proliferation studies
CFSE is a fluorogenic reagent which is frequently used in cell labeling and cell proliferation procedures. Non-fluorescent and moderately hydrophobic in its diesterified form, the dye easily penetrates lipid bi-layers of living cells and is quickly converted to the amine-reactive, green fluorescent form (CSFE) upon cleavage of the acetate groups by intracellular esterases. This highly fluorescent form is membrane-impermeant and is retained on and within the cells, while any excess probe is easily washed away in subsequent wash steps. Because CFSE forms a strong bond inside the cell, it is retained within the cell indefinitely and is inherited by daughter cells. It will not be incorporated into adjacent cells.
Product Information
Product Information
Shipping & Returns
Shipping & Returns
CFSE Green Fluorescent Stain - for cell labelling and proliferation studies
CFSE Green Fluorescent Stain - for cell labelling and proliferation studies
CFSE is a fluorogenic reagent which is frequently used in cell labeling and cell proliferation procedures. Non-fluorescent and moderately hydrophobic in its diesterified form, the dye easily penetrates lipid bi-layers of living cells and is quickly converted to the amine-reactive, green fluorescent form (CSFE) upon cleavage of the acetate groups by intracellular esterases. This highly fluorescent form is membrane-impermeant and is retained on and within the cells, while any excess probe is easily washed away in subsequent wash steps. Because CFSE forms a strong bond inside the cell, it is retained within the cell indefinitely and is inherited by daughter cells. It will not be incorporated into adjacent cells.
Product Information
Product Information
Shipping & Returns
Shipping & Returns
Description
CFSE is a fluorogenic reagent which is frequently used in cell labeling and cell proliferation procedures. Non-fluorescent and moderately hydrophobic in its diesterified form, the dye easily penetrates lipid bi-layers of living cells and is quickly converted to the amine-reactive, green fluorescent form (CSFE) upon cleavage of the acetate groups by intracellular esterases. This highly fluorescent form is membrane-impermeant and is retained on and within the cells, while any excess probe is easily washed away in subsequent wash steps. Because CFSE forms a strong bond inside the cell, it is retained within the cell indefinitely and is inherited by daughter cells. It will not be incorporated into adjacent cells.
