Assay Diluents are additive components used in an ELISA that equalize any differences between the sample matrices (serum, plasma, urine, cell culture fluid) and the calibrator diluent used to generate the standard curve of the ELISA. ICT’s Assay Diluent Optimization Pack, containing four 100 mL ELISA development buffers (General Assay Diluent, IgM-Reducing Assay Diluent, Assay Diluent - Non-Mammalian (formerly Neptune Assay Diluent), and Antigen-Down Assay Diluent), offers an economical method of addressing matrix effects and optimizing signal-to-noise ratio in ELISA development projects.